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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Cancer Res. 2009 Jun 1;69(11):4827–4834. doi: 10.1158/0008-5472.CAN-08-4416

Fig. 3. γ-H2AX foci and Micronucleus Induction.

Fig. 3

A.Percent positive cells for γ-H2AX foci in peripheral leukocytes. Presence of double strand breaks was confirmed by immunofluorescence of γ-H2AX. Positive cells contained >4 distinct nuclear foci. Image caption: Positive and negative cell for nuclear foci, 100X magnification. At least 125 cells were analyzed per sample. Data are represented as mean ± SEM, n=10 per treatment group. **: p<0.01, *: p<0.05 B. Micronucleus induction in peripheral normochromatic erythrocytes. At least 4000 normochromatic erythrocytes were counted and scored for presence of micronuclei. Data are represented as mean ± SEM of micronucleated normochromatic erythrocytes (MN-NCE) per 1000 NCEs. Image caption: MN-NCEs and NCEs, 100X magnification. **: p<0.01, *: p<0.05, by nonparametric one way ANOVA with Dunn’s multiple comparison test. ANOVA of normal linear regression showed effect of treatment, cycle of treatment and interaction of effect of treatment and cycle of treatment to be significant (p<0.01).