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. 2000 Sep 26;97(20):10769–10774. doi: 10.1073/pnas.97.20.10769

Figure 3.

Figure 3

CXR binds to the CYP2H1 264-bp PBRU. (A) Sequences of the wild-type DR-4 element within the 264-bp PBRU and the double mutant with knocked-out hexamer half-sites as adapted from ref. 19. (B) Gel-mobility-shift assay. Radiolabeled 264-bp PBRU was incubated with in vitro transcribed/translated CXR (lane 3–7), chicken RXRγ (lanes 2 and 4–7), anti-RXR antibody (lane 5), unlabeled 264-bp PBRU in 20-fold molar excess (lane 6), and unlabeled 264-bp PBRU double mutant in 20-fold molar excess as competitors (lane 7), as indicated. Arrows depict the unbound probe (c), the complex of CXR, RXRγ, and the probe leading to a shift (b), and the complex of CXR, RXRγ, anti-RXR antibody, and the probe resulting in a supershift (a).