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. Author manuscript; available in PMC: 2009 Jul 14.
Published in final edited form as: Biochemistry. 2008 Feb 7;47(9):2814–2825. doi: 10.1021/bi702457c

Figure 3. The lpxO mutant HG002 lacks lipid A species containing 2-hydroxymyristate.

Figure 3

ESI/MS spectra of crude lipid A species isolated from the wild-type S. typhimurium 14028s (A) or from the lpxO::kan mutant HG002 (B). The minor peaks shown in magneta in panel A are proposed to arise from the following covalent modifications or adducts of the parent compound with [M–2H]2− at m/z 905.6: [M+C16:0–2H]2− at m/z 1024.7; [M+Na–3H]2− at m/z 916.6; [M–2CH2–2H]2− at m/z 891.6; and [M–3OHC14–2H]2− at m/z 792.5.