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. Author manuscript; available in PMC: 2009 Jul 14.
Published in final edited form as: Biochemistry. 2008 Feb 7;47(9):2814–2825. doi: 10.1021/bi702457c

Figure 5. Dependence of LpxO product formation on time and protein concentration.

Figure 5

Increasing amounts of LpxO membrane protein were added to the assay cocktail, and the formation of hydroxylated lipid A was monitored with time. Membrane protein concentrations (mg/ml) in the final assay mixture are indicated. Diamonds, 0.1 mg/ml; squares, 0.05 mg/ml; triangles, 0.025 mg/ml; X, 0.01 mg/ml; stars, 0.005 mg/ml; open circles, no enzyme control. The small amount of apparent product formation at time 0 and in the no enzyme control is caused by slight streaking of the substrate band during TLC, which migrates just above the hydroxylated product band (Fig. 4B).