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. Author manuscript; available in PMC: 2009 Jul 14.
Published in final edited form as: Biochemistry. 2008 Feb 7;47(9):2814–2825. doi: 10.1021/bi702457c

Table 1.

Bacterial strains and plasmids used in this study.

Strain/Plasmid Description Source
Escherichia coli
 XL1-BlueMR Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Stratagene
BLR(DE3)pLysS T7-lac expression strain, recA Novagen
WBB06 ΔrfaC rfaF::tet (76)
Salmonella typhimurium
14028s virulent wild-type S.I. Miller
HG002 lpxO::kan derivative of 14028s (5)
Plasmids
pHSG1 pBluescript II SK containing the lpxO gene (27)
pET21a+ T7-lac expression vector Novagen
pHSG2 pET21a+ containing lpxO gene in NdeI and XhoI sites This work