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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Mol Cancer Ther. 2009 Apr;8(4):893–903. doi: 10.1158/1535-7163.MCT-08-1078

Figure 2.

Figure 2

Cytotoxic effect of the combination of cisplatin and TW-37 on endothelial cells and head and neck tumor cells. A–D, The cytotoxicity of cisplatin and/or TW-37 on human dermal microvascular endothelial cells (HDMEC), oral squamous cell carcinoma-3 (OSCC3), and two University of Michigan squamous cell carcinoma cell lines (UM-SCC-1 and UM-SCC-74A) was determined by SRB assay. Cells were exposed to the IC50, or to one lower and one higher, concentration of cisplatin and/or TW-37 for 72 hours. Combinatorial Index (CI) was calculated in various drug combinations by CalcuSyn software (Biosoft). A synergistic effect, i.e. CI<0.9, is depicted by (■) and an additive effect, i.e. 0.9≤CI≤1.1 is depicted by (▲). In all cases, results are normalized against control and initial plating density. Each panel is representative of at least three independent experiments, performed in triplicate wells per condition.