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. Author manuscript; available in PMC: 2009 Jul 14.
Published in final edited form as: J Neurochem. 1999 Sep;73(3):1164–1174. doi: 10.1046/j.1471-4159.1999.0731164.x

FIG. 1.

FIG. 1

oh8G/oh8dG immunoreactivity after RNase A or 3BR7NI. The fluorescent signal of oh8G/oh8dG in the right (ischemic or ipsilateral) cortices of six animals following FCIR (A—G) and one of the non-FCI (control) animals (H) is shown. Nuclei were counterstained using PI in the presence of RNase A (20 μg/ml, 5 min at room temperature) and appear as red fluorescent signals. The yellowish fluorescent signals indicate colocalization of the oh8dG immunoreactivity with the nuclear PI staining. Two typical cortical samples with 15/0 (A) and 60/0 (B) without reperfusion are shown, along with 90 min of FCI and 15 min of reperfusion (90/15 FCIR in C and D), except the brain samples in D were further treated with RNase A (5 mg/ml,1hat 37°C). A higher magnification is shown of the right cortical sample from one of three animals with 90/15 FCIR and oil (E) or 3BR7NI (F). Another FCIR sample (90/30) is also shown in G. Bar = 30 μm in A—D and 10 μm in E—H.