Figure 6. The MIT domain but not the MTB domain is required for recruitment of spastin to the midbody.

A–C) Myc-spastinΔN194 (A) was not recruited to VPS4(E235Q) structures (B) in the cell body or midbody (box). D–F) 60 kD myc-spastinΔMTB (D) is expressed in a double-ring structure on either side of the stembody, but unlike wild-type 60 kD myc-spastin (Figure S3), does not colocalize with alpha-tubulin (E) throughout the intercellular bridge. Formaldehyde fixation.