Figure 2.
Characterization of IPL-F binding to HPN-expressing cells. A. IPL-F discriminates between HPN expressing prostate cancer cell lines HPN-PC3 and LNCaP and non-expressing cell lines PC3 and DU145. B. IPL-F binds to HPN. Anti-HPN antibody competes for IPL-F binding to HPN-PC3 cells. Green Histogram: HPN-PC3 cells incubated with IPL-F, Gray Histogram: HPN-PC3 cells incubated with anti-HPN antibody and IPL-F. C. Competition assay. IPL-• were competed from HPN-PC3 cells with increasing concentrations of IPL-F whereas ScP was unable to compete for binding at assayed concentrations of peptide