Figure 7. QA treatment decreases the expression of PP1, PP2 and PP5 but increases the expression of PP2B:

20 µg lysate protein (or 20 µl PP2A-IP beads for Figure 7C) was resolved on 10% SDS-PAGE, protein transferred on to PVDF membrane and probed with respective antibodies for each phosphatase. For loading control, the same membranes were stripped and re-probed with anti-actin antibody. For PP2A-IP the band of IgG heavy chain was used as loading control. Graph are mean (±SD) of three experiments (from three separate brains) and are accompanied by a representative blots. Each bar of the quantification graph represents the corresponding band above for each phosphatase.