Table I.
Binding and kinetic data for different ternary complexes in the decoding cycle
| aa-tRNA (anticodon) | Kd (nM) | K(ribosome)1/2 (μM | kmaxGTP (s-1) | kpep (s-1) |
|---|---|---|---|---|
| Phe (GAA) | 1.5 (0.42) | 1.4 (0.68) | 56 (11) | 1.5 (0.49) |
| Val (GAC) | 1.5 (0.29) | 0.99 (0.12) | 38 (4.6) | 1.4 (0.16) |
| Val (UAC) | 1.2 (0.16) | 1.5 (0.72) | 48 (15) | 1.6 (0.22) |
| Gly (GCC) | 1.7 (0.42) | 1.2 (0.80) | 43 (11) | 1.1 (0.44) |
| Glu (UUC) | 1.0 (0.20) | 1.7 (0.37) | 77 (8.3) | 2.6 (0.13) |
| Lys (UUU) | 1.3 (0.33) | 1.3 (0.25) | 60 (4.2) | 2.2 (0.49) |
| Ala (GGC) | 1.7 (0.14) | 1.9 (0.25) | 45 (7.8) | 2.0 (0.20) |
| His (GUG) | 1.4 (0.36) | 1.3 (0.62) | 38 (8.0) | 1.5 (0.46) |
| Ile (GAU) | 2.1 (0.25) | 1.1 (0.38) | 40 (5.1) | 2.3 (0.50) |
| Leu (CAA) | 1.3 (0.30) | 1.0 (0.38) | 41 (6.2) | 2.4 (0.34) |
Numbers in parentheses are standard error of the mean. Kd and kpep values are based on at least 3 independent replicates and K(ribosome)1/2 and kmaxGTP are determined from curves fit to at least 6 apparent kGTP values at different ribosome concentrations.