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. 2009 May 20;29(20):6700–6709. doi: 10.1523/JNEUROSCI.0233-09.2009

Figure 5.

Figure 5.

Bcl-w transcription is ERK5 and MEF2-dependent. A, Schematic of bcl-w 5′ region. Sequence and locations of consensus MEF2-binding sites are indicated. The luciferase construct consisted of the 2.5 kB promoter region, followed by the necleotides ATG and luciferase reporter gene. B, Luciferase reporter assay using bcl-w promoter-Luc cotransfected with pTK–Renilla–Luc for normalization. Two days after transfection by nucleofection, neurons were stimulated with neurotrophins for 2 h. Bcl-w-luciferase expression is neurotrophin dependent. C, D, Bcl-w promoter is ERK5 and MEF2 dependent. Middle and right luciferase reporter assay using bcl-w promoter-Luc, 3xMRE-Luc, or basic Luce cotransfected into COS cells with Renilla luciferase and the indicated expression plasmid(s). Three days after transfection, luciferase activity was measured. Data are from three independent experiments, each of which was conducted in triplicate, and show means ± SEM, *p < 0.05.