. 2009 May 11;48(23):5054–5056. doi: 10.1021/bi900164d

Table 1. Kinetic Parameters for the N-Acetyl Amino Acid-Stimulated ATP−PP_i Exchange Reaction by the MBP−PhsA−His₆ Protein in Its Apo and Holo Forms.

substrate	recombinant PhsA form	k_cat (min⁻¹)	K_m (μM)	k_cat/K_m (μM⁻¹ min⁻¹)	relative activity of endogenous enzyme (7)
(±)-AcPT	apo	87.9 ± 2.9	26.9 ± 2.9	3.27 ± 0.37	74%
	holo	83.5 ± 2.0	23.7 ± 1.9	3.52 ± 0.29
l-AcDMPT	apo	122.9 ± 2.9	198 ± 18	0.62 ± 0.06	100%
	holo	124.7 ± 6.5	254 ± 44	0.49 ± 0.09
N-AcGlu	apo	25.0 ± 1.1	349 ± 55	0.0717 ± 0.011	11%
	holo	28.8 ± 1.5	493 ± 82	0.0584 ± 0.010

Table 1. Kinetic Parameters for the N-Acetyl Amino Acid-Stimulated ATP−PPi Exchange Reaction by the MBP−PhsA−His6 Protein in Its Apo and Holo Forms.