Table 1. Results of incubation of LctM-T405A with peptide sequences attached to the leader peptide of LctA as depicted in Figure 1, panel b.
Entry | Sequence | No. of phosphorylations | Reaction time | Time for full dehydration by wt LctM |
---|---|---|---|---|
1 | RWVRSALLI | 1 | 30 min | <30 min |
2 | RLIKTFAYV | 1 | 1 h | <30 min |
3 | RLIKSFAYV | 1 | 30 min | <30 min |
4 | GHAGSAPPA | 1 | 3 h | |
5 | RLLRSDLVP | 1 | 2 h | |
6 | RPDESLNRL | 1 | 2 h | <30 min |
7 | LAYPSLRLL | 1 | 30 min | <30 min |
8 | ILILSVPVA | 1 | 30 min | <30 min |
9 | FAFYSCPVH | 1 | 30 min | |
10 | LCLCSALLC | 1 | 30 min | |
11 | CYCVSQGPA | 1 | 30 min | <30 min |
12 | LRRASVA | 1 | 1 h | <30 min |
13 | LASPLVNSHV | 2 | 4 h | |
14 | HPSPARPSDA | 1 | 6 h | |
15 | PLSLPALSRA | 2 | 1 h | <30 min |
16 | ARSLGAQSVL | 2 | 4 h | <30 min |
17 | RLIKSFAYVRLLRSLDVP | 2 | 1 h | <30 min |
18 | RLIKSFAYVHAIKSLRNRGRQLSKIPA | 3 | 4 h | |
19 | RLIKSFAYVRLLRSLDVPVARISHNKA | 3 | 4 h |