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. 2009 Jul 21;4(7):e6308. doi: 10.1371/journal.pone.0006308

Figure 4. Long-Term Spermatogenic Potential of Donor Spermatogonia.

Figure 4

(Left) Graph showing relative numbers of Round and Elongating Spermatids in seminiferous tubules of non-transplanted, non-busulfan-treated, Wildtype and DAZL-deficient rat lines at 4 months of age (see Fig. 1a), in comparison to Spermatid numbers in busulfan-treated, DAZL-deficient recipient rats at 212 days (i.e. ∼8 months of age) after being transplanted with rat spermatogonial line, RSGL-GCS9, at passage 13 (i.e. culture day 158). Cell counts were normalized/1000 Sertoli cells. +/−SEM, n = 3 rats/group. (Right) Images of histological sections of seminiferous tubules from the DAZL-deficient recipient rats described in the “Left” panel after being transplanted with spermatogonia from RSGL-GCS9. Bottom Right shows a higher magnification image within the boxed region of the Top Right panel. Scale bars = 100 µm.