Table 1. Progeny from Wildtype and DAZL-Deficient Recipient Rats Transplanted with GCS-EGFP Rat Spermatogonia.
| Recipient | Recipient Background | Culture Passage Number | Days to Analysis | Gram wt per R, L testis | Cells per R, L testis | Days to first litter1 | Number Litters | Average Litter Size | Total Pups Born | Pups Born from Transplanted Stem Cells2 (%)3 |
| Group 1 | ||||||||||
| R946 | Wildtype | p13 | 212 | 0.569, 0.341 | 50,000∶0 | 127 | 3 | 11.3 | 34 | 3 (8.8) |
| R948 | Wildtype | p13 | 212 | 0.735, 0.278 | 50,000∶0 | 131 | 3 | 10 | 30 | 11 (36.7) |
| R949 | Wildtype | p13 | 212 | 1.054, 0.201 | 50,000∶0 | 113 | 3 | 17.3 | 52 | 2 (3.8) |
| Average | p13 | 212 | 0.786, 0.273 | 50,000∶0 | 123.7 | 3 | 12.9 | 38.7 | 4.3 (16.4) | |
| Group 2 | ||||||||||
| R942 | DAZL-Def | p13 | 212 | 0.572, 0.286 | 50,000∶0 | 154 | 3 | 8.7 | 26 | 26 (100) |
| R943 | DAZL-Def | p13 | 212 | 0.651, 0.377 | 50,000∶0 | 141 | 3 | 7.7 | 23 | 23 (100) |
| R944 | DAZL-Def | p13 | 212 | 0.511, 0.326 | 50,000∶0 | 156 | 3 | 8 | 24 | 24 (100) |
| Average | p13 | 212 | 0.578, 0.330 | 50,000∶0 | 150 | 3 | 8.1 | 24.3 | 24.3 (100) | |
| Group 3 | ||||||||||
| R988 | DAZL-Def | p17 | 236 | 0.523, 0.578 | 150,000∶150,000 | 122 | 3 | 12.7 | 38 | 38 (100) |
| R989 | DAZL-Def | p17 | 236 | 0.489, 0.439 | 150,000∶150,000 | 128 | 3 | 9.3 | 28 | 28 (100) |
| R990 | DAZL-Def | p17 | 236 | 0.487, 0.371 | 150,000∶150,000 | 136 | 3 | 5.3 | 16 | 16 (100) |
| Average | p17 | 236 | 0.499, 0.427 | 150,000∶150,000 | 128.6 | 3 | 9.1 | 27.3 | 27.3 (100) |
Wildtype or DAZL-deficient (DAZL-def) recipient rats were transplanted with either 0.5 or 1.5×105 EGFP+ cells/testis from rat spermatogonial line GCS9 at 12 days after busulfan treatment (i.e. 12 mg/kg i.p.) on postnatal day 24. At ∼75 days post-transplantation recipients were paired with 75–80 day old wildtype female rats. Spermatogonia line GCS9 was harvested from passages number 13 and 17, which corresponded to 158 and 204 days in culture, respectively, prior to their transplantation. Recipients R942-R949 were littermates born from a hemizygous, transgenic DAZL-deficient female and a wildtype Sprague Dawley male. No progeny were born from breeder pairs of un-transplanted, busulfan-treated DAZL-deficient males and wild-type females (n = 3 breeder pairs). Breeder pairs of untreated, wild-type male litter mates of DAZL-deficient rats and wild-type female rats from Harlan, Inc. produced 15.5±4.5 pups/litter (+/−SEM, n = 8 litters from 3 breeder pairs).
p = 0.0209 Average Group 1 versus Average Group 2; p = 0.0251 Average Group 2 versus Average Group 3.
p = 0.0031 Average Group 1 versus Average Group 2.
Percent GCS-EGFP + F1 progeny.