Table 1.

Transformation of NIH 3T3 cells by Cotransfection of pFRK44, an Expression Plasmid Encoding gp145^trkB, with Expression Plasmids Encoding BDNF, NT-3, and NGF

Cotransfection of pFRK44 DNAa with			Transforming Activity (Foci per 1.5 × 105 Cells)
Neurotrophic Factor	Plasmidb	DNA (ng)
–	–	–	0
BDNF	pLL42	2000	TMTCc
		200	TMTC
		20	>500
		2	80
NT-3	pLL43	2000	200
		200	44
		20	6
		2	0
NGF	pLTRSNGF	2000	0
		200	0

^aTwo micrograms of pFRK44 DNA was used in each case.

^bTransfection of 2 μg of either pLTRSNGF, pLL42, or pLL43 alone did not induce morphological transformation of NIH 3T3 cells.

^cTMTC, too many to count.