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. Author manuscript; available in PMC: 2009 Jul 14.
Published in final edited form as: Science. 1999 Feb 19;283(5405):1180–1183. doi: 10.1126/science.283.5405.1180

Fig. 4.

Fig. 4

Wnt proteins are sufficient to induce NT-3 expression. Mesenchymal hindlimb tissue (without ectoderm) isolated from E10.5 NT-3lacZ heterozygous embryos was cocultured with aggregates of NIH 3T3–derived cell lines for 24 hours, after which they were stained with X-Gal. (A) Wnt-4–expressing NIH 3T3 cells induce NT-3 in limb mesenchyme. (B) A Wnt-4/limb/Wnt-4 explant stained and sectioned shows two blue stripes at the junction of the two tissues. (C) NIH 3T3 cells or (D) Wnt-5a– expressing cells do not induce NT-3, whereas (E) Wnt-7a– or (F) Wnt-7b–expressing cells induce NT-3 to a certain extent. Arrows point to junctions of limb mesenchyme and cell aggregates. Bar, 0.5 mm.