Figure 3.—
A subset of S288C est2Δ rad52Δ or tlc1Δ rad52Δ cells escapes senescence in liquid culture and exhibits telomeric tract recombination. (A and B) Cells were isolated from a fresh dissection plate (isolates 1–3), inoculated into YPD media, grown to saturation (1–2 × 108 cells/ml), and diluted to 105 cells/ml every 48 hr. At each serial dilution, genomic DNA was extracted for telomere analysis. (C) Telomere Southern blot of S288C haploids with the indicated genotype, as shown in B, during serial propagation in liquid culture every 2 days. Genomic DNA was isolated from cells and digested with XhoI. The membrane was hybridized to a telomeric probe. Marker sizes are indicated at left in kilobase pairs.