Figure 4.—

Adr1^c suppresses cat8Δ-associated defects in Adr1-dependent gene expression. Cell growth is described in materials and methods. RT–qPCR was performed and analyzed as described in materials and methods. Strains were wild type (wt) CKY13 (adr1Δ) and cat8Δ CKY23 (adr1Δ cat8Δ), each carrying either wild-type ADR1 (pKD16) or ADR1-S230A (Adr1^c, pKD14). Derepression was for 4 hr in 0.05% glucose–3% glycerol. The mRNA values were normalized to ACT1 mRNA and are expressed as the percentage of the mRNA level in the wild-type CAT8 strain with Adr1^c as the activator. The values represent the mean ± one standard deviation of three biological replicates assayed in duplicate.