Figure 3.—

Reactivation of GFP expression by exogenous Gal4-VP16. (A) Mock-injected larvae from c269 GFP^off intercrosses show no GFP labeling (n = 100). (B) GFP^off siblings injected with ∼25 pg of plasmid DNA encoding the EF1α ubiquitous promoter driving Gal4-VP16 expression are extensively labeled with GFP (98%; n = 60). (C) DNA from injected or mock-injected larvae was subjected to bisulfite sequencing, and the methylation status of each CG site was reported as a percentage of the total sites tested for 10 clones. Methylated sites are solid circles. Data are representative of two biological replicates.