Figure 4.—

Golgi-ER retrieval is perturbed in some Kar2p retention mutants. (A) Subcellular localization of Erd2p-GFP was examined in each of the Kar2p retention mutants. Unlike the ER localization seen in WT cells, a number of mutants accumulated Erd2p-GFP in punctate foci. In ilm1Δ cells, Erd2p-GFP accumulated in distended ER structures (arrowheads). (B) These Erd2p localization mutants show a variety of interactions, including phenotypic enhancement or synthetic lethality (PE/SL; blue), phenotypic suppression (PS; red), yeast 2-hybrid (Y2-H; green), and physical association (gray). (C) Subcellular localization of the tail-anchored protein, GFP-Frt1p, in WT cells and Kar2p retention mutants.