TABLE 2.
Oligonucleotides used in this study
| Name | Sequence | Description |
|---|---|---|
| PC1 | 5′-GCC GAG CAT AAA GAA GCA-3′ | Forward primer of nsdC |
| PC26 | 5′-CGA ACA GTC CCC TAC AGG-3′ | Reverse primer of nsdC |
| PC101a | 5′-CGC TAg CAT GAC TTC GAT CTA T-3′ | Base changed forward primer of nsdC with NheI site |
| PC102a | 5′-GTG GCC GCT AGc AAC ATA TTA C-3′ | Base changed reverse primer of nsdC with NheI site |
| veAup1 | 5′-CGT ACT CCT ATC CAT CGC-3′ | Forward primer of veA |
| veAlow1 | 5′-GTC GAA CGT ATA GGC AGG-3′ | Reverse primer of veA |
a
Underlined sequences are NheI restriction enzyme sites. Lowercase letters indicate in vitro-mutagenized nucleotides.