Table 2.

Oligonucleotide primers used to amplify the rps4, trnS–trnG, atpB–rbcL, psbA–trnH, trnL intron and ITS with amplification direction and reference

Primer	Sequence	Direction	Reference
rps4
rps5	5′-ATGTCCCGTTATCGAGGACCT-3′	Forward	Souza-Chies et al. (1997)
trnS	5′-TACCGAGGGTTCGAATC-3′	Reverse	Souza-Chies et al. (1997)
trnS–trnG
trnS (GCU)	5′-GCCGCTTTAGTCCACTCAGC-3′	Forward	Hamilton (1999)
trnG (UCC)	5′-GAACGAATCACACTTTTACCAC-3′	Reverse	Hamilton (1999)
atpB–rbcL
atpB-1	5′-ACATCKARTACKGGACCAATAA-3′	Forward	Chiang et al. (1998)
rbcL-1	5′-AACACCAGCTTTRAATCCAA-3′	Reverse	Chiang et al. (1998)
psbA–trnH
trnHR	5′-CGCGCATGGTGGATTCACAAATC-3′	Forward	Tate (2002)
psbAF	5′-GTTATGCATGAACGTAATGCTC-3′	Reverse	Sang et al. (1997)
trnL
trnL-c	5′-CGAAATCGGTAGACGCTACG-3′	Forward	Taberlet et al. (1991)
trnL-d	5′-GGGGATAGAGGGACTTGA AC-3′	Reverse	Taberlet et al. (1991)
ITS
ITS4	5′-TCCTCCGCTTATTGATATGC-3′	Forward	Swensen et al. (1998)
ITS5p	5′-GGAAGGAGAAGTCGTAACAAGG-3′	Reverse	Swensen et al. (1998)