Table 1.
Characteristics of detected proteins and antibodies used in the experiment, together with the dilutions applied
| Protein | Mol. weight (kD) | Primary antibody | Secondary antibody (alkaline-phosphatase conjugated) |
|---|---|---|---|
| COR14b | 14·4 | Rabbit anti-COR14b raised against a synthetic polypeptide, AgriSera, Vännäs, Sweden | Goat anti-rabbit IgG (whole molecule), Sigma1 : 50 000 |
| 1 : 20 000 | |||
| SPS (sucrose phosphate synthase) | 130 | Chicken anti-SPS, AgriSera | Rabbit anti-chicken IgY (IgG) (whole molecule), Sigma 1 : 50 000 |
| 1 : 40 000 | |||
| RbcL (RuBisCO large subunit) | 58 | Chicken anti-RbcL (global antibody), AgriSera | |
| 1 : 20 000 | |||
| PsbS | 22 | Chicken anti-PsbS (global antibody), AgriSera | |
| 1 : 40 000 | |||
| PsbA (D1) | 32 | Chicken anti-PsbA (global antibody), AgriSera | |
| 1 : 20 000 |