Table 2.

Primer combinations, annealing temperature (T_a), percentage polymorphism, total and average band number used for IRAP

Primer combination	Ta	Total bands	Polymorphic bands	Percentage polymorphism	Band size (bp)	Maximum band number		Minimum band number		Average bands in Ae. tauschii*	Average bands in T. aestivum*
LTR6150 + 3′LTR	62·6	18	18	100	310–1450	At536	11	At531	2	7 ± 1·7	6·5 ± 0·7
LTR6149 + Sukula	47	9	9	100	200–1200	At1	9	At25	1	5 ± 0·5	2
LTR6150 + Nikita	59·5	16	15	93·7	180–3100	At526	10	Several accessions	1	5·5 ± 2·8	11·5 ± 2·1
LTR6150 + Sukula	62·3	35	35	100	320–3100	At524	16	At407	5	10·9 ± 2·5	14 ± 1
						At544		At408
5′LTR2 + Sukula	65·6	34	34	100	320–3200	At535	19	At20	5	13·3 ± 3·4	6·5 ± 0·7
						At532
5′LTR2 + 3′	66	17	17	100	240–2500	At25	8	Several accessions	1	3·5 ± 2·1	5 ± 2·8
3′LTR + RTY1	54·9	16	16	100	310–1050	At28	11	At537	1	6·4 ± 2	4
								At538	2
3′LTR + 3′LTR	64	26	25	95·3	200–1600	At9	14	At25	6	10·1 ± 2·1	11·5 ± 0·7
Total		171	169	98·8						7·85 ± 3·31	8·12 ± 3·7

* ± s.d.