Table 2.
Mean red fluorescence (channel number) of PI-stained 2C nuclei of green leaf and red bract of poinsettia and leaf of pea measured either alone or in co-chopped mixtures using an unknown variety on day 1 and known varieties on days 4–8
| Material |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Alone |
Co-chopped mixtures |
Ratio |
||||||||
| Poinsettia |
Pea + Poinsettia |
Pea + Poinsettia |
||||||||
| Day and replicate | Pea | Leaf | Bract | Poinsettia, Leaf + Bract | Pea | Leaf | Pea | Bract | Pea/leaf | Pea/bract |
| 1·1 | 826·3 | 255·7 | 130·9 | – | 763·0 | 262·5 | 572·9 | 189·2 | 0·344 | 0·330 |
| 3·1 | 764·9 | 235·1 | 177·5 | – | 707·1 | 272·6 | 582·2 | 201·9 | 0·386 | 0·347 |
| 3·2 | 782·8 | 245·3 | 161·9 | – | 725·6 | 282·0 | 587·6 | 211·2 | 0·389 | 0·359 |
| 3·3 | 782·3 | 278·6 | 202·0 | – | 722·3 | 271·3 | 585·8 | 207·9 | 0·376 | 0·355 |
| 3·4 | 790·5 | 274·0 | 185·6 | – | 722·7 | 263·7 | 586·1 | 205·5 | 0·365 | 0·351 |
| Mean | 789·4 ± 10·1 | 257·7 ± 8·3 | 171·6 ± 12·1 | – | 728·1 ± 9·3 | 270·4 ± 3·5 | 582·9 ± 2·7 | 203·1 ± 3·8 | 0·372 ± 0·008 | 0·348 ± 0·005 |
| 4·1 | 772·4 | 294·1 | 224·6 | 216·2 | 760·4 | 277·4 | 691·3 | 248·2 | 0·365 | 0·359 |
| 4·2 | 806·9 | 265·0 | 219·5 | 195·6 | 755·5 | 289·0 | – | – | 0·383 | – |
| 4·3 | 719·3 | 265·1 | 224·8 | 165·9 | 762·3 | 282·7 | 725·7 | 265·9 | 0·371 | 0·366 |
| 6·1 | 800·9 | 263·6 | 179·2 | 246·5 | 758·4 | 285·9 | 696·3 | 259·9 | 0·377 | 0·373 |
| 6·2 | 842·5 | 292·1 | 215·9 | 232·2 | 737·8 | 272·9 | 635·3 | 228·9 | 0·370 | 0·360 |
| 6·3 | 810·1 | 287·7 | 208·6 | 246·6 | 791·3 | 297·7 | 765·1 | 281·0 | 0·376 | 0·367 |
| 7·1 | 780·5 | 285·6 | 220·8 | 223·9 | 724·1 | 262·3 | 619·4 | 218·9 | 0·362 | 0·353 |
| 7·2 | 795·7 | 284·1 | 214·4 | 226·8 | 764·4 | 284·8 | 661·0 | 233·6 | 0·373 | 0·353 |
| 7·3 | 793·0 | 288·5 | 245·4 | 242·7 | 778·0 | 287·0 | 787·3 | 287·1 | 0·369 | 0·365 |
| 8·1 | 794·0 | 277·7 | 234·6 | 249·7 | 816·7 | 306·3 | 691·5 | 251·1 | 0·375 | 0·363 |
| 8·2 | 817·8 | 283·1 | 223·8 | 240·2 | 717·1 | 261·4 | 659·6 | 238·8 | 0·365 | 0·362 |
| 8·3 | 850·4 | 304·2 | 234·1 | 230·1 | 789·9 | 295·9 | 664·3 | 240·0 | 0·375 | 0·361 |
| Mean | 798·6 ± 9·8 | 282·6 ± 3·7 | 220·5 ± 4·7 | 226·4 ± 7·1 | 763·0 ± 8·2 | 283·6 ± 3·9 | 690·6 ± 15·6 | 250·3 ± 6·5 | 0·372 ± 0·002 | 0·362 ± 0·002 |
| Overall mean* | 795·9 ± 7·4 | 275·3 ± 4·4 | 206·1 ± 7·3 | 226·4 ± 7·1 | 752·3 ± 7·4 | 279·7 ± 3·3 | 657·0 ± 16·7 | 235·6 ± 7·2 | 0·372 ± 0·003 | 0·358 ± 0·002 |
* Overall means are all significantly different (P < 0·001). Mean values are given with the standard deviation.
Note, the poinsettia/pea ratio for green leaf is 0·372 and for red bract 0·358 with a mean of 0·365. The mean gives a DNA amount for poinsettia of 4C = 7·10 pg (1C = 1·76), close to the figure given previously in Bennett et al. (2000) of 4C = 6·6 pg (1C = 1·65 pg), assuming Pisum sativum ‘Minerva Maple’ 4C = 19·46 pg (1C = 4·87) (Bennett and Smith, 1976). The genome size obtained using green leaf is 4C = 7·23 pg (1C = 1·81), compared with 7·04 pg (1 C = 1·74) obtained using red bract, a significant difference (P < 0·001) of 4·0 %.