Table 1.

Primers used for PCR analysis of rol and aux genes (Ri plasmid) in 55 Coffea arabica hairy root clones established after A. rhizogenes-mediated transformation

Genes	Primers	PCR product size (bp)	Reference
rolA	5′-ACGGTGAGTGTGGTTGTAGG-3′	403	Slightom et al. (1986)
	5′-GCCACGTGCGTATTAATCCC-3′
rolB	5′-GCTCTTGCAGTGCTAGATTT-3′	383	Furner et al. (1986)
	5′-GAAGGTGCAAGCTACCTCTC-3′
rolC	5′-CTCCTGACATCAAACTCGTC-3′	586	Furner et al. (1986)
	5′-TGCTTCGAGTTATGGGTACA-3′
rolD	5′-TGCCTTGAGGTCATTCATCAAGGCC-3′	741	Slightom et al. (1986)
	5′-ATGGACTGAAGGAGCACTCATTGGC-3′
aux1	5′-CTCCGATTCCTTTCCAACCG-3′	791	Camilleri and Jouanin (1991)
	5′-CGCACGTTATCCTCATACCC-3′
aux2	5′-CTGTCAACGGAGGCTGTTGGG-3′	722	Camilleri and Jouanin (1991)
	5′-ACCCTAGTCTCATCCCAGGG-3′