Table 4.
PCR analysis of aux and rol genes insertion in coffee hairy root clones. The presence of the different aux and rol genes belonging to the TR and TL T-DNA sub-fragments from the A. rhizogenes Ri plasmid was determined in 55 hairy root clones
| Genes from TL |
Genes from TR |
|||||||
|---|---|---|---|---|---|---|---|---|
| Hairy root clones | rolA | rolB | rolC | rolD | aux1 | aux2 | Phenotypic alteration | |
| A4RS strain (control) | + | + | + | + | + | + | ||
| Non-transformed roots | − | − | − | − | − | − | ||
| X1 | + | + | + | + | − | − | − | |
| X2 | + | + | + | + | − | − | − | |
| X3 | + | + | + | + | − | − | Thick roots | |
| X4 | + | + | + | + | − | − | − | |
| X6 | + | + | + | + | − | − | − | |
| X8 | + | + | + | + | − | − | − | |
| X9 | + | + | + | + | − | − | − | |
| X11 | + | + | + | + | − | − | − | |
| X12 | + | + | + | + | − | − | − | |
| X13 | + | + | + | + | − | − | − | |
| X14 | + | + | + | + | − | − | − | |
| X16 | + | + | + | − | − | − | Fast growing | |
| X17 | + | + | + | + | − | − | − | |
| X18 | + | + | + | + | − | − | − | |
| X19 | + | + | + | + | − | − | Fast growing | |
| X20 | + | + | + | + | − | − | − | |
| X22 | + | + | + | − | − | − | − | |
| X23 | + | + | + | + | − | − | − | |
| X24 | + | + | + | + | − | − | − | |
| X25 | + | + | + | + | − | − | − | |
| X26 | + | + | + | + | − | − | − | |
| X28 | + | + | + | + | − | − | Thick roots | |
| X29 | + | + | + | − | − | − | − | |
| X30 | + | + | + | + | − | − | Thick roots | |
| X32 | + | + | + | + | − | − | − | |
| X34 | + | + | + | + | − | − | − | |
| X36 | + | + | + | + | − | − | − | |
| X37 | + | + | + | − | − | − | − | |
| X38 | + | + | + | − | − | − | − | |
| X39 | + | + | + | − | − | − | Thick roots | |
| X40 | + | + | + | − | − | − | − | |
| X41 | + | + | + | + | − | − | − | |
| X43 | + | + | + | + | − | − | Thick roots | |
| X44 | + | + | + | + | − | − | − | |
| Y3 | − | + | + | + | − | − | − | |
| Y5 | + | + | + | + | − | − | − | |
| Y8 | + | + | + | + | − | − | − | |
| Y9 | + | + | + | + | − | − | − | |
| Y12 | + | + | + | + | − | − | − | |
| Y13 | + | + | + | + | − | − | − | |
| Z1 | + | + | + | + | − | − | Thick roots | |
| Z3 | + | + | + | + | − | − | − | |
| Z5 | − | + | + | − | − | − | − | |
| Z7 | + | + | + | + | − | − | − | |
| Z8 | − | + | + | − | − | − | − | |
| Z10 | − | + | + | + | − | − | Thick roots | |
| Z14 | + | + | + | + | − | − | − | |
| Z15 | + | + | + | + | − | − | − | |
| Z16 | + | + | + | + | − | − | − | |
| Z18 | − | + | + | + | − | − | Thick roots | |
| Z19 | − | + | + | + | − | − | − | |
| Z21 | + | + | + | + | − | − | − | |
| Z22 | − | + | + | − | − | − | − | |
| Z23 | + | + | + | − | − | − | − | |
| Z24 | + | + | + | + | − | − | − | |