Figure 2.

Human xenografts exhibit robust ER and PR expression.

Xenograft in an ovariectomized mouse without any hormonal treatment shows robust expression of ER by IHC (A). ER expression is down-regulated after E2 treatment (B). Negative IHC control with normal mouse (C) and rabbit (F) IgG. PR IHC of a xenotransplant in a mouse treated with E2 (D) demonstrates edema associated with E2 treatment and up-regulation of PR as compared with ovariectomized control (E). Brown staining indicates positive cells; two replicate experiments were conducted with same results. Original magnification ×100.