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. 2009 Jul 15;15(9-10):297–306. doi: 10.2119/molmed.2009.00030

Figure 1. siRNA effects on the protein and mRNA levels of the respective targets.

Figure 1

A) Two siRNAs for each target gene were delivered to CSMCs from donor 1 by Cellfectin and the protein levels evaluated three days after transfection; representative blots are reported; B) the most effective siRNA among each couple were delivered by Metafectene and the protein levels evaluated (CSMCs from donor 1), shown are representative blots; C) cumulative data obtained from both Cellfectin and Metafectene transfection are indicated; data, reported as mean ± SEM, n = 5, were normalized to GAPDH and expressed as ratio of siGL2 treatment, #p< 0.02 compared to control; D) target mRNA levels were evaluated after siRNA delivery either by Cellfectin (CSMCs from donor 1 and 2) or E) Metafectene (CSMCs from donor 2); 28S transcript levels served as normalizator; NTC = non treated cells, siGL2 = control siRNA treated cells, data are expressed as mean ± SEM, n = 4. * p< 0.001 and + p< 0.05 compared to control.