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. 2009 Jul 15;15(9-10):297–306. doi: 10.2119/molmed.2009.00030

Figure 3. Time course effects of the selected siRNAs on CSMC proliferation.

Figure 3

A) Inhibition of CSMC proliferation, as evaluated by BrdU incorporation, lasted up to day five post transfection; the kinetic of cell proliferation reduction for siRNA treated cells was mathematically evaluated by equation 1 (black lines); cumulative data from both donors are expressed as mean ± SEM, n = 6; B–D) proliferation inhibition was paralleled by a concomitant reduction of target proteins: shown are representative blots from donor 1 performed using Cellfectin as transfectant; # all the numbers reported below the blots derive from band quantification normalized to GAPDH and are expressed as ratio of siGL2 treatment; * p<0.02 compared to controls, n=4; NTC = non treated cells, siGL2 = control siRNA treated cells.