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. 2009 Jul 15;15(9-10):297–306. doi: 10.2119/molmed.2009.00030

Figure 5. Interrelation among E2F1, cyclin E1 and E2 in CSMCs.

Figure 5

Three days after trasfection, each of the target gene was depleted by the respective siRNAs and the protein levels (A) of the other two target genes evaluated: in the upper panel representative blots out of three independent experiments are reported (CSMCs from donor 2), in the lower panel the total data are shown (CSMCs from donors 1 and 2); # all the numbers reported below the blots are derived from band quantification normalized to GAPDH and are expressed as ratio of siGL2 treatment; § protein levels were reduced at day five post transfection; B) the mRNA levels corresponding to the data shown in A) are reported (28S transcript levels served as normalizator); cumulative data from both donors are expressed as mean ± SEM, n = 6. * p< 0.05 compared to control.