Table 1.

ΔG_HX values for the slowly exchanging amide protons at 0.0 M GdmCl, 30°C, pD 6.8 (pD_read + 0.4) in D₂O

Residue number	kex, s−1*	kint, s−1†	ΔGHX, kcal/mol
19	2.83  × 10−7	7.3	10.3
50	4.27  × 10−7	8.5	10.1
52	1.63  × 10−7	3.0	10.1
98	1.77  × 10−7	2.8	10.0
Average			10.1

It should be noted that Clarke and Fersht (9) obtained higher ΔG_HX values. Obviously, they used different k_int. An independent check of the k_int values can be made easily by using the program on the web site http://dino.fold.fccc.edu:8080/sphere.html. 

^*Values of k_ex are from Clarke and Fersht (9). 

^†Values of k_int are from Bai et al. (15).