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. Author manuscript; available in PMC: 2009 Jul 14.
Published in final edited form as: J Chromatogr A. 2007 Oct 10;1172(2):127–134. doi: 10.1016/j.chroma.2007.09.075

Figure 2.

Figure 2

Reconstructed ion chromatogram illustrating resolution of 23 of 24 metabolite standards (10 μM of each metabolite) using ternary gradient of formic acid, ammonium formate and acetonitrile. Standards were mixed together before analysis. The LC column used was 30 cm long × 50 μm I.D. packed with Atlantis dC-18 3 μm particles and equipped with an integrated nanospray tip with 3 μm diameter. Gradient is given in the experimental section. Flow velocity was 100 nl/min. * indicate peak for the analyte when multiple peaks are detected.