Table 1.
Figures of merit for metabolite standards. % RSDs were analyzed based on peak height for 5 μM n = 3. Lower limits of detection were calculated as the concentration that would give a S/N of 3 based on the average S/N ratio of peaks at sample concentrations of 5 μM, n = 3. Detection limits were determined using gradient separation conditions similar to those used in Figure 2.
| Metabolite | % RSD | LLOD (fmol) | LLOD (μM) |
|---|---|---|---|
| G6P/F6P | 24 | 4 | 0.5 |
| F1,6P | 18 | 6 | 0.6 |
| Glyceraldehyde-3-Phosphate | 16 | 30 | 4 |
| 2/3 Phospho-glycerate | 26 | 30 | 4 |
| Bis-phospho-glycerate | 17 | 7 | 0.8 |
| Phosphoenol Pyruvate | 11 | 20 | 3 |
| Pyruvate | 33 | 20 | 2 |
| Acetyl CoA | 25 | 2 | 0.2 |
| Citrate | 31 | 10 | 1 |
| Isocitrate | 30 | 7 | 0.8 |
| α-Ketoglutarate | 18 | 10 | 1 |
| Succinyl CoA | 30 | 4 | 0.4 |
| Succinate | 10 | 9 | 1 |
| Fumarate | 27 | 10 | 2 |
| Malate | 19 | 3 | 0.3 |
| Oxaloacetate | 24 | 20 | 2 |
| ATP | 6 | 5 | 0.6 |
| ADP | 10 | 4 | 0.4 |
| NAD | 17 | 1 | 0.1 |
| GTP | 19 | 5 | 0.6 |
| GDP | 34 | 4 | 0.4 |
| FAD | 32 | 2 | 0.2 |