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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Virology. 2009 Jun 11;390(2):279–288. doi: 10.1016/j.virol.2009.05.017

Figure 4. Removal of PML protein enhances JCV infection.

Figure 4

SVG-A cells were treated with media (no treatment), 1uM arsenite, 3uM arsenite, or 500IU/mL INF-β at indicated time points. Treated cells were infected with JCV and infection was scored 48 hrs later using indirect immunofluorescence of JCV LT. (A) Positive cells were quantified and the data represent an average of three infections compared to no treatment (set at 100%). There is a drastic increase in infection when samples are pre-treated with arsenite. Conversely, there is a decrease in infection when samples are treated with INF-β. There is no effect when samples are treated 24 hrs post-infection. Error bars represent the standard deviation of the samples. (B) Representative indirect immunofluorescence images of JCV LT 48 hrs post-infection. Samples were mounted on slides with DAPI medium.