Table 1.
Lifetimes, apparent dissociation constants, and apparent open pair lifetimes in r(G·C) base-pairs of the 16-mer and the 11-mer hairpins at 15°C
[Mg2 + ] |
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τopen (ns) | ||
---|---|---|---|---|---|
16-mer simplex | G546·C | 0 eq | 4.8 (3.4, < < 1)a | 21 (2.2, 20)a | 101 (7.5, < < 20)a |
1 eq | 6.5 | 7.6 | 49 | ||
3 eq | 11 | 15 | 160 | ||
G553·C | 0 eq | 22 (33, 7)b | 1.6 (0.28, 0.57)b | 35 (9.2, 4)b | |
1 eq | 41 | 4.0 | 160 | ||
3 eq | 43 | 2.2 | 95 | ||
11-mer simplex | G572·C | 0 eq | 11 (3.4, < < 1)a | 4.9 (2.2, 20)a | 54 (7.5, < < 20)a |
1 eq | 13 | 9.5 | 120 | ||
3 eq | 11 | 11 | 120 |
The uncertainties on base-pair lifetimes are estimated as ±30%. The uncertainties on dissociation constants are about ±20%
aAs a comparison, the parameters measured for base-pair (G2· C) respectively in [r(CGCGAUCGCG)]2 and [d(CGCGAUCGCG)]2 are given inside parentheses
bThe parameters measured for base-pair (C3· G) respectively in [r(CGCGAUCGCG)]2 and [d(CGCGAU CGCG)]2 are given inside parentheses