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. Author manuscript; available in PMC: 2009 Jul 15.
Published in final edited form as: Virology. 2007 Jan 16;361(2):316–324. doi: 10.1016/j.virol.2006.11.031

FIG. 1.

FIG. 1

Analysis of the purity of the nuclear fraction obtained from HSV-1-infected cells. Approximately 2 x 106 Vero cells were infected with HSV-1 at a MOI of 10 pfu/cell. At 15 h after infection, the cells were harvested and separated into the cytoplasmic and nuclear fractions as described in the Methods. The proteins of the nuclear and cytoplasmic fractions were resolved by SDS-PAGE followed by Western blot analysis simultaneously using antibodies to the nuclear marker lamin B1 (70 kDa) and the endoplasmic reticulum marker calnexin (90 kDa) to assay the same nitrocellulose blot.