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. Author manuscript; available in PMC: 2009 Jul 15.
Published in final edited form as: Virology. 2007 Jan 16;361(2):316–324. doi: 10.1016/j.virol.2006.11.031

FIG. 6.

FIG. 6

Rate-zonal centrifugation of capsids isolated from cells infected with the VP23-negative mutant (K23Z) and analysis for the presence of tegument proteins VP1/2 and UL37. Vero (non-complementing cell line) and C32 (complementing cell line expressing VP23) cells were infected with the VP23-negative mutant virus, K23Z, at a MOI of 10 pfu/cell. At 15 h after infection the capsids from the nuclear fraction were obtained, separated by rate-zonal centrifugation, analyzed by SDS-PAGE followed by Western blot analysis as described in the legend to Fig. 2. The same blot was probed sequentially for the presence of VP5 and tegument proteins VP1/2 and UL37.