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. 2000 Sep 26;97(20):10814–10819. doi: 10.1073/pnas.97.20.10814

Figure 1.

Figure 1

Colocalization of Rad51 and Dmc1 foci. Spreads of meiotic nuclei were prepared and indirectly immunostained with antibodies against Rad51 (green; Left) and Dmc1 (red; Right). Single-staining images are pseudocolored from the gray scale images. Merged images are a two-channel combination of original image pairs (Middle). Representative nuclei are shown. (A) WT, 3 h; (B) red1, 3 h; (C) tid1, 3 h; (D) rad54 tid1, 3 h; (E) red1 tid1, 3 h; (F) rad54 tid1, 12-h nucleus showing Rad51 aggregates; (G) WT, 4.5-h nucleus with mostly D foci; (H) rad54, 4.5-h nucleus with mostly R foci; (I and J) WT, 4.5-h nucleus showing side-by-side R and D foci; (K and L) rad54 tid1, 7.5-h nucleus showing side-by-side R and D foci. J and L are photographic enlargements of the areas indicated in I and K, respectively. (White scale bars = 2 μm long.)