Table 1.
Double staining analysis of Rad51 and Dmc1 in various mutants
| Strain | Number of foci per
nucleus at 3 h*
|
% of RD foci† | Time of entry, h‡
|
Life span, h§
|
||||
|---|---|---|---|---|---|---|---|---|
| RD foci | R foci | D foci | R+ stage | D+ stage | R+ stage | D+ stage | ||
| WT | 60 ± 18 | 6.7 ± 2.9 | 11 ± 2.8 | 77 (3.8) | 2.5 | 2.5 | 1.6 | 2.1 |
| red1 | 13 ± 4.1 | 16 ± 3.4 | 11 ± 4.8 | 32 (2.9) | 3.5 | 3.5 | 2.1 | 2.5 |
| tid1 | 43 ± 22 | 44 ± 15 | 59 ± 21 | 30 (11) | 2.6 | 2.5 | 6.9 | 8.5 |
| rad54 | 43 ± 16 | 14 ± 2.4 | 13 ± 5.9 | 61 (3.7) | 3.0 | 3.0 | 3.1 | 1.7 |
| rad54 tid1 | 22 ± 9.9 | 47 ± 17 | 93 ± 31 | 13 (12) | 2.3 | 2.3 | NA | NA |
| red1 tid1 | 14 ± 4.1 | 35 ± 12 | 47 ± 19 | 15 (13) | 3.0 | 2.6 | 3.4 | 5.1 |
| zip1 | 64 ± 3.2 | 5.8 ± 1 | 13 ± 5.8 | 77 (ND) | ND | ND | ND | ND |
ND and NA, not determined, and not available, respectively.
*
For each strain, at least 100 unselected 3-h meiotic nuclei were counted. Numbers are averages of three to six independent experiments. Experimental errors are also indicated as ±.
†
The predicted frequency of fortuitous superposition, as estimated by the computer program dot-stat (37), is given in parenthesis.
‡
Time at which 50% of nuclei had entered the focus-positive stage, as determined from the cumulative curves in Fig. 2C.
§
Life spans of focus-positive stages as determined from the noncumulative curve data in Fig. 2B.