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Maturation of dendritic cells (DCs) transduced with recombinant carcinoembryonic antigen (CEA) or Tat-CEA proteins. Expression of co-stimulatory molecules and I-Ab on DCs were analysed after 16 h of incubation with recombinant proteins or lipopolysaccharide (LPS). DCs were stained with antibodies against with I-Ab, CD40, CD86, CD80 and CD11c. Live DCs (7AADneg/CD11cpos) were analysed for the surface molecule expression using a FACS Canto II flow cytometer.
