Fig. 5.

Carcinoembryonic antigen (CEA)-specific cytotoxic lymphocytes from mice immunized with dendritic cells (DCs). DCs (1 × 10⁶ cells) pulsed with either CEA or Tat-CEA proteins were injected twice at weekly intervals to B6 mice (three mice per group). At 7 days post-second immunization, splenocytes were harvested and restimulated with CEA proteins for 3 days to generate effector cells. Effector cells were incubated with target cells (either MC38 or MC38-CEA2 cells) for 18 h and washed away. The remaining target cells on the culture plate were labelled with [³H]-methylthymidine (5 µCi) for 3 h. Specific lysis (%) was determined by the measurement of [³H]-methylthymidine uptake. Representative data from three independent experiments are presented as mean ± standard error of triplicate cultures. *Statistically significant at P < 0·05 using at a Student's t-test compared with other groups.