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. Author manuscript; available in PMC: 2010 May 29.
Published in final edited form as: Chem Biol. 2009 May 29;16(5):473–478. doi: 10.1016/j.chembiol.2009.04.007

Table 1.

Activity of amino acid adenylating enzymes.

Enzyme Amino Acid %γ–18O4-ATP exchange
TycA D-Phe 100 ±2.5
L-Phe 69.8 ±7.8
ORF21a HA 8.9 ± 2.3
MHA 27.3 ± 3.2
ValAa Val 5.5 ± 0.8
TrpRSa Trp 9.7 ± 1.6
LysRSa Lys 13.0 ± 2.7
a

Exchange measured by MALDI-TOFMS for a panel of amino acids. Exchange is only listed for active amino acids. All other amino acids tested fell below the threshold of detection for the MALDI-TOFMS based assay.