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. 2009 Jun 24;106(28):11600–11605. doi: 10.1073/pnas.0902626106

Fig. 3.

Fig. 3.

Rescue of cell viability after withdrawal of growth factors. (A and B) Cells transfected with GRP94-mGFP shown before (A, Left panels) and 40 h after serum withdrawal (B, Right panels). Bright field and fluorescence images of the same fields, showing transfected and untransfected cells under both conditions. Magnification 200×. (C) Quantitation of survival of cells cotransfected with cytosolic GFP and GRP94-mCherry. Cells expressing GFP alone, or GRP94-mCherry alone, or both constructs were scored at the indicated times after the shift to serum-free medium, and their percentage of viability is plotted. (D) Quantitation of total fluorescence per transfected cell. Grp94−/− cells expressing WT GRP94-mGFP were incubated in serum-free medium and photographed at the indicated times. For quantitation, cells were binned into brightly fluorescent at t = 0 (squares), medium fluorescent (triangles), and dull fluorescent cells (circles). Total pixel intensity per cells was calculated with the SlideBook software and is presented as means ± SD of 10–21 cells per time point.