Fig. 2.

The complex formed by TRPP2 and PKD1 C-terminal interacting domains has 3 TRPP2 and 1 PKD1. Bar graph compares the calculated and measured molecular masses (determined by static light scattering) of the indicated proteins. The calculated molecular mass was obtained according to the stoichiometry schematized on the right. The protein fragments (indicated at the bottom) were tagged with either hexahistidine (His₆), MBP, or His₆ plus SUMO. MBP and SUMO also served to increase the resolution and accuracy of the molecular mass measurement. MBP-His₆ was used as a control to demonstrate the accuracy of the equipment. Results are shown as mean and SD. n = number of measurements.