Figure 1.

Characterization of protein species in lymphocyte extracts detected by the sandwich ELISA antibodies, mouse monoclonal and chicken polyclonal anti-FMRP. Western blots of lymphocyte extracts, 2 μg/lane from eight individuals with CGG repeats in the normal range. Films were overexposed to demonstrate a minor, cross-reacting protein species (asterisk) larger than 100 kDa to the chicken antibody (A), which is not detected by the mouse antibody (B). Mouse anti-glyceraldehyde-3-phosphate dehydrogenase shows sample loading (C).