Figure 6.

The sandwich ELISA is a reliable tool for the measurement of FMRP. Lymphocyte extracts from individuals were aliquoted and stored at −80°C before assay. A: A set of 15 samples were assayed on two separate occasions, comparison of the measurements shows the reproducibility of the ELISA. B: Another set of three samples was measured in three separate experiments over a 6-month period. Values relative to an internal reference standard (fiducial lymphocyte extract) that was included in each experiment were calculated. Repeated measurement of FMRP in lymphocyte protein extracts yielded reproducible results. CV = coefficient of variation. C indicates control; FF1 and FF2 indicate full-mutation females 1 and 2. Blackened bars indicate FMRP measurement with first ELISA, light gray bars indicate second ELISA, and dark gray bars indicate third ELISA.