. 2009 Jul;11(4):334–346. doi: 10.2353/jmoldx.2009.080151

Table 4.

Allelic Loss Observed in Multiplex Panel

Multiplex panel tested	Homozygous DNA sample tested	Observed allelic loss (of either allele)	Cause	Solution
Panel 1	c.−81A>G (detectable by Panel 2)	c.−80T>C	Homozygous c.−81A>G causes a strong mismatch at the 3′ end of an extension primer for c.−80T>C	DNA sequencing focusing on c.−80T>C
Panel 2	c.19G>A (Hb C) and c.20A>T (Hb S) (detectable by both Panels 1 and 2)	c.27_28insG	Homozygous c.19G>A and c.20A>T result in a mismatched site at the 9^th and 8^th base respectively from 3′ end of primer for c.27_28insG whose length is only 14 bases. The primer is furthermore at a disadvantage in competition for the same template region with the other two for c.19 and c.20	DNA sequencing focusing on c.27_28insG or rechecking it by exclusion of primers for c.19G>A and c.20A>T from Panel 2
	c.92 + 5G>C (detectable by Panel 1)	c.92 + 1G>T	Homozygous c.92 + 5G>C has a base mismatch at the 4^th base from 3′ end of a primer for c.92 + 1G>T	DNA sequencing focusing on c.92 + 1G>T
Panel 3	c.19G>A (Hb C) (detectable by both Panels 1 and 2)	c.20A>T (Hb S)	Homozygous c.19G>A has a mismatch at 3′ end of extension primer for c.20A>T (only in this panel)	c.20A>T (Hb S) can be detected in Panel 1 and 2 without this interference (different primer is used)